Protein_Domain
Part:BBa_K2356000:Design
Designed by: Ralf Philipsen Group: iGEM17_TU-Eindhoven (2017-10-19)
CT33 with mCherry and Strep-tag II
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 22
Illegal BamHI site found at 751 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 772
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added multiple restriction sites, so that the 3 parts may be exchanged. CT33 could for example be exchanged for a CT52 construct using SalI and SacI enzymes. We also altered the DNA sequences in order to optimize expression in E. coli.
Source
mCherry is a genomic sequence first discovered in mushroom anemones. Strep-tag II is only 8 amino acids long and experimentally proven by others to bind to Streptavidin. The CT33 part is derived from the genomic DNA coding for H+-ATPase, a protein which is present in many organisms.