Part:BBa_K2355001
Cleavable peptide linker for Bitis arietans and Bitis gabonica venom
Peptide linker that is cleaved by Bitis arietans and Bitis gabonica venom.
When translated, this linker should contain 5 x repeats of the ALK motive identified by our experiments to be cleavable by the venom of Bitis sp.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Results of experiments
We tested the AMC-substrate peptide against the venom from our three snakes of interest, Bitis arietans, Bitis gabonica and Naja nigricollis. We made measurements in five different timepoints, with five different venom concentrations. The background noise was deducted from all measurements. The experiment showed that this particular substrate is cleaved significantly by the two venoms from Bitis arietans and Bitis gabonica, but not by the venom of Naja nigricollis as seen in figures 2-4. Different concentrations of venom had great effect in the fluorescence intensity.
Figure 1: ALK-AMC incubated with different concentrations of Bitis arietans venom.
Figure 2: ALK-AMC incubated with different concentrations of Bitis gabonica venom.
Figure 3: ALK-AMC incubated with different concentrations of Naja nigricollis venom.
In conclusion we were able to detect a significant difference from the Bitis species and the Naja nigricollis.
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