Composite

Part:BBa_K2350022:Design

Designed by: CHIA-SUI CHIANG   Group: iGEM17_NYMU-Taipei   (2017-10-23)


pPIGBACK-PrbcL-CrtZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used fusion PCR to fuse CrtZ and PrbcL to avoid restriction site in between which might influence expression efficiency.

Source

pPIGBACK is from BBa_K2350009, and CrtZ is from Pantoea ananatis.

References