Composite

Part:BBa_K2350019:Design

Designed by: PIN-YU CHEN   Group: iGEM17_NYMU-Taipei   (2017-10-21)


R0010-B0034-Holin-B0010-B0012


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

　　Because the gene sequences of endolysin and holin in different species of bacteriophages are different, we are not sure whether endolysin and holin from different species could cooperate well. Therefore, we choose the endolysin and the holin both are from enterobacteria phage T4.

Primer

  1. p0011 　　5' tatttctcctctttctctag 3'
  2. p0012 　　5' ctagtatttctcctctttctctag 3'
  3. p0017 　　5' cgctgatagtgctagtgtagatcg 3'
  4. p0018 　　5' gatagtgctagtgtagatcg 3'
  5. p0019 　　5' ctagatggcagcacctagaatatc 3'
  6. p0020 　　5' atggcagcacctagaatatc 3'
  7. p0021 　　5' agcgttatttagcccttcctaata 3'
  8. p0022 　　5' ttatttagcccttcctaata 3'

Source

　　The holin we used is from iGEM released parts, BBa_K112000, which is holin from enterobacteria phage T4.

References

  • Yibo Shi, Yaxian YanEmail author, Wenhui Ji, Bin Du, Xiangpeng Meng, Hengan Wang and Jianhe Sun. (2012). Virology Journal 2012 9:70.
  • 2014 Peking Team: http://2014.igem.org/Team:Peking/Suicide
  • UniPort: http://www.uniprot.org/uniprot/P06808
  • Wikipedia: https://en.wikipedia.org/wiki/Holin