Composite

Part:BBa_K2350018:Design

Designed by: PIN-YU CHEN   Group: iGEM17_NYMU-Taipei   (2017-10-21)


J23106-B0034-Endolysin-B0010-B0012


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 334
    Illegal AgeI site found at 404
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

　　Because the gene sequences of endolysin and holin in different species of bacteriophages are slightly different, we are not sure whether endolysin and holin from different species could cooperate well. Therefore, we choose the endolysin and the holin both are from enterobacteria phage T4.

Primer

  1. p0009 　　5' agagccaggcatcaaataaa 3'
  2. p0010 　　5' tactagagccaggcatcaaataaa 3'
  3. p0011 　　5' tatttctcctctttctctag 3'
  4. p0012 　　5' ctagtatttctcctctttctctag 3'
  5. p0013 　　5' ctagatacttaggaggtattatga 3'
  6. p0014 　　5' atacttaggaggtattatga 3'
  7. p0015 　　5' agtagctttatagatttttatacg 3'
  8. p0016 　　5' gctttatagatttttatacg 3'

Source

　　The endolysin we used is from iGEM released parts, BBa_K112806, which is endolysin from enterobacteria phage T4.

References

  • Mathias Schmelcher, David M Donovan, and Martin J Loessner. (2012). Bacteriophage endolysins as novel antimicrobials. Future Microbiol. 2012 Oct; 7(10): 1147–1171. doi: 10.2217/fmb.12.97
  • 2014 Peking Team: http://2014.igem.org/Team:Peking/Suicide
  • UniPort: http://www.uniprot.org/uniprot/P00720
  • Wikipedia: https://en.wikipedia.org/wiki/Lysin