Composite

Part:BBa_K2350017:Design

Designed by: YI-CHIEN LIN   Group: iGEM17_NYMU-Taipei   (2017-10-22)


J23118-B0034-NrtA-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1240
    Illegal BamHI site found at 610
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we want the NrtA be produced continuously,so we choose a moderate constitutive promoter.


Source

Synechocystis sp. PCC 6803 genomic DNA

References