Part:BBa_K2350000:Design

MelA gene coding sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 909
  Illegal XhoI site found at 31
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1103
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 187
  Illegal BsaI.rc site found at 478
  

Design Notes

The sequence has been codon optimized to express efficiently in cyanobacteria.

Source

The DNA sequence is obtained from genome of nitrogen-fixing bacterium Rhizobium etli.