Coding
Part:BBa_K2349002:Design
Designed by: Eka Rusadze, Luka Bulatovic Group: iGEM17_Tartu_TUIT (2017-10-06)
SUC2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 787
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1510
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1088
Illegal SapI.rc site found at 415
Design Notes
Addition of prefix and suffix and removal of illegal restriction site in the codon of amino acid 277 from T to G
Source
Amplified from genomic DNA of S. cerevisiae CENPK 21C with primers GAATTCGCGGCCGCTTCTAGATGCTTTTGCAAGCTTTCCTTTTCC and CTGCAGCGGCCGCTACTAGTACTATTTTACTTCCCTTACTTGGAACTTGTCAATG