Composite

Part:BBa_K2342006:Design

Designed by: Ushanandini Mohanraj   Group: iGEM17_Aalto-Helsinki   (2017-10-25)


DCD1L peptide linked to sumo fusion peptide with His6x tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BglII site found at 410
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed such that the DCD-1L peptide could be produced in E.coli without killing the host. So Smt3 fusion tag was added to the DCD-1L. Further for effective purification of DCD-1L after expression, His tag was added. Hence design was based on effective production and purification of the desired peptide DCD-1L.


It is codon optimized for E. coli.

Source

The part encodes for a human peptide. It is chemically synthesized and obtained as double-stranded DNA fragment, gBlock from IDT. (https://eu.idtdna.com/pages/products/genes/gblocks-gene-fragments)

References