Tag
Part:BBa_K2342001:Experience
Designed by: Ushanandini Mohanraj Group: iGEM17_Aalto-Helsinki (2017-10-25)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2342001
Employing of Smt3 tag is beneficial in several aspects in expression of recombinant proteins. Ulp1 is a robust enzyme that specifically facilitates cleavage of Smt3, leaving protein of interest unbound to tag. SUMO tag is known for its effect on solubility and preventing inclusion bodies of fusion peptide, significantly easing purification step.
For more details on the production and usage of this part please check the composite part page: BBa_K2342006, BBa_K2342007, BBa_K2342008, BBa_K2342009
User Reviews
UNIQae5703a2dc02476b-partinfo-00000000-QINU UNIQae5703a2dc02476b-partinfo-00000001-QINU