RBS

Part:BBa_K2340008

Designed by: Laurens Heling   Group: iGEM17_Kent   (2017-10-31)


Guide RNA construct of human Pkp4 (2)

Pkp4 guide RNA construct with a human U6 promoter and human U6 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 183
    Illegal XhoI site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This product will be translated by RNA polymerase III to form a guide RNA. This small RNA strand can be processed by the CRISPR dCas13a from Leptotrichia buccalis (LbuC2c2; BBa_K2340000) to form a CRISPR RNA (crRNA).

The sequence that is complementary to Human Pkp4 is ACATTTCCTCACTTCCCTGA. This is one of two Pkp4 guide RNA constructs we have constructed


DNA for this part has not been submitted as a sample to the registry due to time restraints.


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Categories
Parameters
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