Coding

Part:BBa_K2336031:Design

Designed by: Ziyang Xiao   Group: iGEM17_HUST-China   (2017-10-14)


oprF-GS-FLAG-LBT8-GS-LBT8-GS-LBT8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 72
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
    Illegal AgeI site found at 915
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 816


Design Notes

We amplificated the oprF and FLAG-LBT separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes.

Source

Synthesized by IDT.And we fused 2 sequences(oprF and 3*LBT).

References