Part:BBa_K233306:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K233306

This is essentially an export tag and must be fused upstream of the gene of the protein that is to be secreted out of the cell into the medium.

The fusion must be in-frame so as to get a functional protein-of-interest. Once this is ensured the tag helps in directing the fusion protein( i.e. of the tag and the protein-of-interest)to the membrane bound Tat. The Tat (twin-arginine translocation) system delivers folded proteins across biological membranes 1 YcdB is the signal sequence that is used in this pathway.

User Reviews

UNIQ86748ab802e0b5b0-partinfo-00000000-QINU UNIQ86748ab802e0b5b0-partinfo-00000001-QINU