Reporter
Pblind-GFP

Part:BBa_K2332001:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-09-27)


Blue light inducible expression system with GFP reporter (Pblind GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 709


Design Notes

Pblind promoter was designed by Jayaraman P. et al. (2016)

Source

GFP sequence was obtained from: BBa_E0040 The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References

Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005.