Composite
Co-op
Part:BBa_K233002:Design
Designed by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil Group: iGEM09_IBB_Pune (2009-07-10)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3918
Design Notes
A suitable export tag was synthesizd and added in frame. the export tag was designed based on the twin arginine translocation (tat) pathway from E.coli
Source
source- biobricks torA,lacZ, pLac - eschercihia coli GFP- aequoria victoria