Composite

Part:BBa_K233001:Design

Designed by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil   Group: iGEM09_IBB_Pune   (2009-07-10)


Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4099


Design Notes

a suitable export tag was synthesizd and added in frame. the export tag was designed based on the twin arginine translocation (tat) pathway from E.coli


Source

source- biobricks torA,lacZ, pLac - eschercihia coli GFP- aequoria victoria


References