Composite
Part:BBa_K2328040:Design
Designed by: Zhongyi Jiang Group: iGEM17_TJU_China (2017-10-14)
HU + smURFP III + Histag.a + RBS III + HO-1 III + pMB1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 840
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 840
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 840
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 840
Illegal NgoMIV site found at 330
Illegal NgoMIV site found at 407 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2256
Design Notes
It is a co-expression construction used in B. longum. But since B.longum is a kind of obligate anaerobe, this part is just a test of our project (whether B.longum NC2700 is oxygen resistence or not).
Source
AAAA