Device

Part:BBa_K2325104:Design

Designed by: Xiaojia Liu   Group: iGEM17_SCUT-FSE-CHINA   (2017-10-18)


N20-sgRNA function device in pTarget


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 41


Design Notes

Source

The N20 sequence selected from the genome of T7 phage. Overlap extension of synthetic oligonucleotides

References