Part:BBa_K2325103:Design

An expression device for fusion protein of Formamidase and Phosphite dehydrogenase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 821
  Illegal NgoMIV site found at 1308
  Illegal NgoMIV site found at 1503
  Illegal NgoMIV site found at 1650
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 159
  

Design Notes

Mutagenesis to remove internal PstI site.