Coding

Part:BBa_K2325000:Design

Designed by: Wenyong Lou   Group: iGEM17_SCUT-FSE-CHINA   (2017-10-14)


Formamidase (FOR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 69
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutagenesis to remove internal PstI site.


Source

cloned from the genome of Paenibacillus pasadenensis. This sequence have been accepted as a patent owned by Mr. Wenyong Lou.

References