Composite

Part:BBa_K2324006:Design

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-16)


pRha_FimH_225sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for expression in E. coli . Research was required to identify the ideal placement of the foreign protein domain.


Source

The CDS is the new part BBa_K2324001. The RBS and terminator are the registry parts BBa_B0034 and BBa_B0015 respectively. The rhamnose-inducible promoter is part BBa_K902065.

References