Coding

Part:BBa_K2323010:Design

Designed by: Aurore Dupin   Group: iGEM17_Munich   (2017-10-27)


Lwa Cas13a coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1286
    Illegal BglII site found at 2966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2365


Design Notes

This is a large protein, and the coding sequence has to be codon-optimized, or expressed with a strain that is enriched in tRNAs for rare codons (such as E.coli Rosetta2). Furthermore, this protein is not very soluble and should be tagged with a solubility tag, such as SUMO or MBP. N-terminal context affects the activity of the protein.


Source

Leptotrichia wadei.

References

Sequence was taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)