Part:BBa_K231000

Metal binding peptide

This part codes for a peptide containing a metal binding motif. De novo design allows this peptide to have high affinity for metals. Affinity derives from Cys-Gly repeats. This peptide encodes two Cys-Gly-Cys-Cys-Gly repeats. Other metals besides arsenic, such as Cd and Hg, should be bound as well. A similar sequence was reported to successfully increase the ability of E. coli to sequester and survive high levels of heavy metals (Pazirandeh, M., Wells, B., Ryan, R. Development of Bacterium-Based Heavy Metal Biosorbents: Enhanced Uptake of Cadmium and Mercury by Excherichia coli Expressing a Metal Binding Motif. Appl Environ Microbiol. 64, 4068-4072 (1998).)

Insertion of this gene into K12 derived E. coli strains did not appear to affect growth in a LB.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]