Composite

Part:BBa_K2309005:Design

Designed by: Haohui Fang   Group: iGEM17_XJTLU-CHINA   (2017-09-29)


Manifestation of AIP induced expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1267
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2325
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is for detection of expression efficiency of agr-regulated system, hence, we combined BBa_K515005 and BBa_2309003 together. Moreover, the sensing system for AIP has been optimized for Lactococcus lactis.

Source

Superfolder GFP is derived from BBa_K515005 and AIP-induced agr-regulated system is from another part of our team, which is numbered BBa_K2309003.

References