DNA

Part:BBa_K2308002:Design

Designed by: Yunpeng Dai   Group: iGEM17_ECUST   (2017-10-04)


sYFP2 knock In for fusion expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 319
    Illegal BamHI site found at 1217
    Illegal XhoI site found at 96
    Illegal XhoI site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 36
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 336
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If the up/downstream sequence in genome is different from the part, the efficiency of homologous recombination will be much lower. There's NO spacer between BBa_K2308008& BBa_K2308003.


Source

The up/downstream sequences are from the genome of Rhodobacter Sphaeroides, and the sYFP2 coding sequence is from Part:BBa_K2308003 .

References