Part:BBa_K2308001:Design

knock out gene crtB on genome

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 93
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 47
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 162
  Illegal SapI site found at 779
  

Design Notes

If the up/downstream sequence in genome is different from the part, the efficiency of homologous recombination will be much lower.

Source

The part is from the genome of Rhodobacter Sphaeroides