Coding

Part:BBa_K2307003

Designed by: Marguerite Batsale   Group: iGEM17_Bordeaux   (2017-10-26)


unc-60 - actin-binding proteins that regulate actin filament dynamics in C.elegans

Unc-60 encodes two, tissue-specific isoforms of actin depolymerizing factor(ADF)/cofilin. These proteins are actin-binding proteins that regulate actin filament dynamics. UNC-60A, primarily binds G-actin, depolymerizes actin filaments, and inhibits actin polymerization. UNC-60B, which does not inhibit actin polymerization, binds F- and G-actin and influences the rate of actin polymerization by the UNC-60B:actin ratio. UNC-60B is required for actin filament organization in body wall muscle, and locomotion. UNC-60A and UNC-60B are differentially expressed: UNC-60A is found in oocytes, embryos, and the adult gonad and intestine, while UNC-60B is found in body wall muscle, the vulva, and spermatheca. unc-60 transcripts undergo muscle-specific pre-mRNA processing, regulated by the ASD-2 and SUP-12 splicing factors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 335
    Illegal XhoI site found at 1024
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None