Composite

Part:BBa_K2306001:Design

Designed by: Gabriella Tany   Group: iGEM17_TUDelft   (2017-10-04)


TorA-GFP (LacI promoter, RBS, double terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 999


Design Notes

To ensure the compatibility of the different parts in our project, we considered several different promotors. In the end, we decided to use the LacI promotor as it is compatible with high-copy plasmids and can easily be inhibited by IPTG.


Source

Combination of BBa_K2306000, BBa_R0010, BBa_B0030 and BBa_B0015.

References