Composite

Part:BBa_K2305015

Designed by: PENG ZHAO   Group: iGEM17_BIT   (2017-10-19)


strong promoter and cycle amplification system

Strong constitutive promoter stars the circuit, Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux and form a positive feedback.

Characterization map

K230500

Fig.The Growth curve of E.coil.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1591
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 64


[edit]
Categories
Parameters
None