Reporter

Part:BBa_K2305010

Designed by: PENG ZHAO   Group: iGEM17_BIT   (2017-09-22)


GFP/RFP dual fluorescent system with LacI and plac

It's a GFP/RFP dual fluorescent system

Agarose gel electrophoresis

We calculated the new part’s base number to be 3134bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500

DNA sequencing[edit] The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

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K230500


Characterization map

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Fig.The Growth curve of E.coil.

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Fig.The GFP curve of E.coil.

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Fig.The RFP curve of E.coil.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2846
    Illegal AgeI site found at 2958
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1844


[edit]
Categories
Parameters
None