Project

Part:BBa_K2305008

Designed by: PENG ZHAO   Group: iGEM17_BIT   (2017-10-19)


pcat promoter and plux,luxr generator

pcat promtor controlled plux and LuxR

Agarose gel electrophoresis

We calculated the new part’s base number to be 928bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500

DNA sequencing

The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

K230500

Characterization map

K230500

Fig.The Growth curve of E.coil.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 64


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