Part:BBa_K2302011:Design

T7-His-Lpp-ompA-L-FABP-T7terminater

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 491
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 716
  

Design Notes

The plasmid had better to transform into E.coli( DE3).

Source

Lpp-OmpA and FABP is synthesis by Genewiz company, and we insert them into plasmid pET-14b.