Composite
Part:BBa_K2302009:Design
Designed by: Hongyang Wei Group: iGEM17_NEFU_China (2017-10-19)
His+Lpp-ompA+L-FABP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 462
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 687
Design Notes
the plasmid is transformed into E. coli (DE3) for protein expression.
Source
Lpp-OmpA and FABP are chemically synthesized, and subcloned into the expression plasmid pET-14b.