Composite

Part:BBa_K2302007

Designed by: Hongyang Wei   Group: iGEM17_NEFU_China   (2017-10-19)


His+PelB+CALB

This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists three basic parts: His, PelB and CALB. PelB is sequence cords for a functional signal peptide, which transport the fused protein CALB out the membrane efficiently, and CALB is a kind of lipase which can hydrolyze fat. By transforming this plasmid, a bacteria can secret lipase out the membrane,hydrolyzing fat in the environment to fatty acids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1050
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 78
    Illegal NgoMIV site found at 771
    Illegal NgoMIV site found at 881
    Illegal AgeI site found at 573
    Illegal AgeI site found at 831
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None