Coding

Part:BBa_K2301001

Designed by: Aaron Renberg   Group: iGEM17_Michigan   (2017-10-27)


T4 Endolysin optimized for expression in E. coli

T4 endolysin is a lysozyme isolated from the T4 bacteriophage that lyses a bacteral cell from the inside. It does this by degrading the peptidoglycan layer of the cell wall, exposing the bacteria to the extracellular environment and killing it. The activity of this enzyme can be improved when T4 holin is also present. We incorporated this part along with BBa_K2301000, BBa_K2301002 and BBa_K2301003 into a temperature controlled kill switch for bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 259
    Illegal AgeI site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None