Coding
Part:BBa_K2300003:Design
Designed by: Ari Edmonds Group: iGEM17_Macquarie_Australia (2017-10-12)
HydEF (Updated 2017)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1849
Illegal NheI site found at 2059
Illegal NheI site found at 2503
Illegal NotI site found at 253 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 901
Illegal AgeI site found at 1816
Illegal AgeI site found at 3459 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2684
Illegal BsaI.rc site found at 304
Illegal BsaI.rc site found at 416
Design Notes
Removal of introns and codon optimisation for E. coli.
Source
Chlamydomnas reinhardtii