Coding

Part:BBa_K2293000:Design

Designed by: Sarah Cooke   Group: iGEM17_Bristol   (2017-10-24)


nitrite reductase cytochrome c-type protein NrfB H89Y


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Unwanted restriction sites were removed by sequence editing. This coding sequence was originally part of a longer operon sequence but due to synthesis difficulties we then designed new primers to extract the gene from the sequence and amplify it.

Source

The source of this part is the wild-type genome of the E. coli strain K.12

References