Coding
comE
Part:BBa_K2292001:Design
Designed by: THUAN-THIEN TCHEN Group: iGEM17_CCU_Taiwan (2017-10-26)
Response regulator of competence regulon, comE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 164
Illegal XhoI site found at 19
Illegal XhoI site found at 49 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is taking from uniProt and codon optimized for B. subtilis
Source
This part was designed from http://www.uniprot.org/uniprot/A0A1W7R3C5
References
1. doi: 10.1038/srep26502 - Membrane Topology and Structural Insights into the Peptide Pheromone Receptor ComD, A Quorum-Sensing Histidine Protein Kinase of Streptococcus mutans
2. doi: 10.1016/j.tim.2004.11.009 - Quorum sensing in streptococcal biofilm formation