Coding
comE

Part:BBa_K2292001:Design

Designed by: THUAN-THIEN TCHEN   Group: iGEM17_CCU_Taiwan   (2017-10-26)


Response regulator of competence regulon, comE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 164
    Illegal XhoI site found at 19
    Illegal XhoI site found at 49
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is taking from uniProt and codon optimized for B. subtilis


Source

This part was designed from http://www.uniprot.org/uniprot/A0A1W7R3C5

References

1. doi: 10.1038/srep26502 - Membrane Topology and Structural Insights into the Peptide Pheromone Receptor ComD, A Quorum-Sensing Histidine Protein Kinase of Streptococcus mutans

2. doi: 10.1016/j.tim.2004.11.009 - Quorum sensing in streptococcal biofilm formation