Part:BBa_K2292001

Response regulator of competence regulon, comE

ComE is a response regulator of competence regulon. It binds to comD-CSP complex be phosphorylated. The phosphorylated comE will bind onto a comE responsive promoter on downstream gene, turn on the transcription of it.

In our project, we replaced the downstream gene with GFP. Below is a picture showing how this part work in our construction

Quorum sensing (QS), is a methodology of bacteria to communicate, just like language to us human. The bacteria learn their population situation by accepting or delivering quorum sensing molecules. Quorum sensing can work on bacteria of the same species and bacteria of different species as well. They can interact with the very specific molecule within same species just like native language; and interact with universal molecule inter-species, just like a universal foreign language.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 164
  Illegal XhoI site found at 19
  Illegal XhoI site found at 49
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]