Part:BBa_K2291001
DaIRIP4 - The Ice Inhibitor
This part includes the coding region from the Ice Recrystallisation Inhibition Protein 4 from Antarctic Hairgrass. This protein binds to growing ice crystals, preventing ice crystal growth and altering ice crystal morphology to prevent the growing crystals causing cellular damage. This part should reduce the damage done by cold stress.
The 2017 SECA NZ team characterised the effectiveness of DaIRIP4 with an electrolyte leakage assay.
DaIRIP4 transgenic plants showed improved frost resistance at 0oC when compared to controls.
Further testing showed some DaIRIP4 transgenic plants were resistant to damage at -20oC (A & B), while others performed no better than controls (C, D, & E).
An interesting result occurred at 25oC, where DaIRIP4 transgenic plants lost far fewer electrolytes than control plants.
More information can be found in the 2017 SECA NZ iGEM Project Results page.
http://2017.igem.org/Team:SECA_NZ/Results.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 640
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 573
| chassis | Arabidopsis thaliana |