Regulatory

Part:BBa_K2287001:Design

Designed by: Ruiling Cai   Group: iGEM17_UCAS   (2017-10-18)


glnAp2 + riboJ + RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

This part is a basic part containing a nitrogen-sensitive promoter glnAp2 and its regulatory sequences. The pre-attached RBS frees users from tedious DNA assembly. The riboJ is added to insulate the transcriptional elements, so that they will not interfere with each other. This enables users to test the function of the promoter more precisely.

Source

The DNA of glnAp2 is synthesized according to documented sequences. RiboJ and RBS comes from Lou lab.

References

1. Reitzer, Lawrence J., and Boris Magasanik. "Transcription of glnA in E. coli is stimulated by activator bound to sites far from the promoter." Cell 45.6 (1986): 785-792.

2. Zong, Yeqing, et al. "Insulated transcriptional elements enable precise design of genetic circuits." Nature Communications 8 (2017).