Coding

Part:BBa_K2286010:Design

Designed by: Demin Xu   Group: iGEM17_UESTC-China   (2017-10-20)


HheC- F186L-W249P: halohydrin dehalogenase site-directed mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is codon-optimized for E.coli.


Source

This gene is from Agrobacterium radiobacterAD1.


References