RNA
Part:BBa_K2285010:Design
Designed by: Zhuoyang Chen Group: iGEM17_SJTU-BioX-Shanghai (2017-10-18)
Antisense of Star system3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have to conserve the stem-loop strucuture of the original sequence.
Source
This system of Star3, including both Target3 and its Antisense3, was originated from the attenuator in T181 strain (Chappell J, Takahashi MK, Lucks JB. 2015. Creating small transcription activating RNAs),and certain modification and improvement were made.