Composite

Part:BBa_K2284003:Design

Designed by: Eleanor Rose Boardman   Group: iGEM17_UNOTT   (2017-10-26)


gRNA 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constructed using BsaI sites to minimise restriction sites in the sequence



Source

Synthesised

References