Device

Part:BBa_K228110

Designed by: Shan Shen   Group: iGEM09_PKU_Beijing   (2009-09-22)

AND GATE AraC + SupD + LacP +RBS(J61100)+T7ptag



This Device belongs to the SupD_T7ptag AND Gate family constructed by Peking University 2009 iGEM Team

PKU AND GATE FOR PARTS.png

This is a AND GATE in the Ecoli stain JM109. Use arabinose to induce the transcription of SupD and use IPTG to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.

This gate is only one of nine gates(K228110-K228118), which are different in the RBS of T7ptag.

For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1323


[edit]
Categories
Parameters
n/aAND GATE AraC + SupD + LacP +RBS(J61100)+T7ptag