RNA

Part:BBa_K228001:Design

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-08-25)


SupD-tRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 33
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36


Design Notes

There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.


Source

SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.

References