Device

Part:BBa_K2277666:Design

Designed by: Geyi Wang   Group: iGEM17_ZJUT-China   (2017-10-24)


A blue-light-sensitive self-lysis system in E.coli cells


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

None.


Source

This part's sequence comes from the following literature:Wu H, Wang Y, Wang Y, Cao X, Wu Y, Meng Z, Su Q, Wang Z, Yang S, Xu W, Liu S, Cheng P, Wu J, Khan MR, He L, Ma G. Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi. PMID:25524104. And the new FixK2 promoter comes from: Expression vector pDawn, complete sequence. GenBank: JN579121.1. The functional gene lysin comes from SCITHERA.

References