Composite
pTet-COMT

Part:BBa_K2276005:Design

Designed by: Zhiwei Luo   Group: iGEM17_SCU_China   (2017-10-23)


pTet and the coding sequneces of caffeic acid o-methyltransferase (COMT) with N-His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 759
    Illegal AgeI site found at 1209
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because of the codon bias can influence the expression of the gene, we optimized the codon of caffeic acid o-methyltransferase (COMT).


Source

The original sequnence of caffeic acid o-methyltransferase (COMT) comes from GenBank (accession no.AK064768). The sample used for experiments was artificially synthesized by Tsingke. What's more,the sequnence of pTet comes from previous part.

References

Byeon Y, Back K. Melatonin production in Escherichia coli, by dual expression of serotonin N -acetyltransferase and caffeic acid O -methyltransferase[J]. Applied Microbiology & Biotechnology, 2016, 100(15):6683.