Composite
pTet-COMT
Part:BBa_K2276005:Design
Designed by: Zhiwei Luo Group: iGEM17_SCU_China (2017-10-23)
pTet and the coding sequneces of caffeic acid o-methyltransferase (COMT) with N-His tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 973
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 759
Illegal AgeI site found at 1209 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because of the codon bias can influence the expression of the gene, we optimized the codon of caffeic acid o-methyltransferase (COMT).
Source
The original sequnence of caffeic acid o-methyltransferase (COMT) comes from GenBank (accession no.AK064768). The sample used for experiments was artificially synthesized by Tsingke. What's more,the sequnence of pTet comes from previous part.
References
Byeon Y, Back K. Melatonin production in Escherichia coli, by dual expression of serotonin N -acetyltransferase and caffeic acid O -methyltransferase[J]. Applied Microbiology & Biotechnology, 2016, 100(15):6683.