Regulatory

Part:BBa_K2275006

Designed by: I-Hsin Chen   Group: iGEM17_NCKU_Tainan   (2017-10-09)

PyeaR(BBa_K216005) with additional NsrR binding site behind B0030 RBS

We add an extra NsrR binding sequence at the downstream of RBS on the original part of K381001 in order to lower the basal level of expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None