Coding

Part:BBa_K2275004

Designed by: I-Tai Shih   Group: iGEM17_NCKU_Tainan   (2017-10-09)

Glutamine synthetase (glnA) with reverse glutamate dehydrogenase (gudB)

We construct gudB and glnA together. It’s more convenient for us to operate them for converting ammonia ion to glutamine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 449
    Illegal BamHI site found at 1083
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 96
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 2228
    Illegal AgeI site found at 352
    Illegal AgeI site found at 2665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1255


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Categories
Parameters
None